Antioxidant properties of extracts derived from placentae of different gestation terms

Abstract

Svitlana Rozanova

Objective: Human placenta extract (HPE) is known to possess antioxidant activity due to a high concentration of bioactive substances. This fact contributes to the perspective of its application in clinical practice in order to treat oxidation-induced diseases. In this study, a comparative investigation of antioxidant properties of HPEs derived from placentas of the 38 weeks gestation terms and post maturity is provided. Methods: Antioxidant activity of aqueous-saline HPEs was evaluated spectrophotometrically using ferric reducing power assay, ABTS+ cation radical decolorization assay, chelating ability assay and total phenolics assay. HPE biological activity was evaluated by ability to decrease methemoglobin formation in a model experiment with erythrocytes under nitrite-induced oxidative stress. Results: Total ABTS+ scavenging and chelating abilities of extracts derived from placenta of 38 weeks gestation term is higher in comparison with full term spontaneous delivery (40 weeks). Ferric reducing power of extracts does not differ regarding gestation term. Treatment of human erythrocytes with HPE of earlier gestation term significantly attenuates a nitrite-induced oxidative damage. However, HPE derived from placenta of 40 weeks gestation (full term) does not possess protective effect against nitrite induced oxidative stress in erythrocytes. Conclusion: Treatment of human erythrocytes with HPEs of earlier gestation term significantly attenuates a nitrite-induced oxidative damage.