Biocompatibility of Osteoblasts in Titanium Morphology Created by Dc Voltage

Abstract

Xiao-quan Mao* and Zhi-ping Zheng

Objective: To investigate the biocompatibility of osteoblasts in titanium morphology that created with micro arc oxidation at different DC voltage.

Methods: The titanium was cut into 10 x 10 x 1 mm³ and they were grind and polished respectively. DC voltage that treated titanium was used single variable control: 200 V, 250 V, 300 V, 350 V, 400 V, 450 V; treatment time: 5S; the treatment temperature was less than 40°C, Electric current and other conditions were same. Then osteoblasts were cultured on titanium morphology.

Results: The number of adhesion cells was higher in the experiment groups in comparison with that in the group Ti that culture for 60 and 120 minutes, the groups MAO350V, MAO400V and MAO450V were significantly higher than Ti (p<0.01). The proliferation of cells in the experiment groups was not obviously changed on first day. But the number of cells of the experiment groups was significantly higher than group Ti after 3 d, 5 d and 7 d. The ALP of cellsin the experiment groups was higher than that in the group Ti at 7 d and 14 d. There were statistically significant between the groups 350 V, 400 V, 450 V and Ti (p<0.01).

Conclusion: It has a good biocompatibility of osteoblasts in titanium morphology.