Correlation of cobalt binding activity of albumin with the common markers of oxidative stress in thalassemia syndrome patients

Abstract

Angshuman De, Neepa Chowdhury, Pradyot Kumar Sen, Indranil Chakraborty,Prantar Chakraborty, Kaushik Bhowmick

Scanty evidence exists for change in cobalt binding activity of albumin in Thalassemia. The aim of the present study was to observe whether cobalt binding activity of albumin would be altered in thalassemia patients receiving chronic transfusion and also whether the same would be correlated with oxidative stress markers. The present hospital-based, non-interventional, cross-sectional case control study was undertaken in Medical College and Hospital, Kolkata, India. Sixty patients with thalassemia syndrome requiring blood or erythrocyte transfusion for more than five years were randomly selected as cases. Age and sex matched controls were selected from apparently healthy individuals who were neither thalassemic trait nor carrier. Cobalt binding activity of albumin (ACB), thiobarbituric acid reactive substances (TBARS), protein carbonyls, protein bound sialic acid/sialoprotein ratio were measured in serum samples. There was no significant difference in cobalt binding activity between case and control. Serum protein carbonyls and TBARS were significantly higher in case population compared to control group. Ratio of serum protein bound sialic acid to serum mucoprotein was significantly lower in thalassemia cases. The study demonstrated that ACB was not correlated with serum TBARS, serum protein carbonyls as well as protein bound sialic acid/sialoprotein ratio in thalassemia. In conclusion, ACB is not superior to other markers of oxidative stress in identifying oxidative stress in thalassemia patients receiving repeated transfusions.