Determination of serum protein desialylation status as an indicator of duration of transfusion therapy in individuals with thalassemia syndrome

Abstract

Mrinal Pal, Indranil Chakraborty, Utpal Chowdhuri,Jayanta De , Kanika Mandi Choudhuri

In thalassemia patients blood transfusion is the mainstay of therapy. Blood transfusion is associated with oxidative stress. Oxidative stress has its influence on plasma proteins. The present study was carried out to explore the level of sialic acid content and oxidative changes in serum proteins of thalassemia subjects on regular blood transfusion. Blood was collected from thalassemia patients receiving long duration of blood transfusion and from newly diagnosed cases of thalassemia who never receive blood transfusion and from carriers or traits of thalassemia as control. The extent of carbonylation and desialylation of serum protein was estimated by dinitrophenylhydrazine and thiobarbuturic acid method respectively. The effects of in vitro treatment of serum protein H2O2 on the above mentioned parameters were observed. Carbonylation of serum protein was significantly higher and sialic acid content of serum protein significantly lower in thalassemia subjects on regular blood transfusion. A significant negative correlation exists between carbonylation and sialic acid content of serum protein. Enhanced desialylation and carbonylation of serum protein by in vitro H2O2 treatment suggest that oxidative stress can cause desialylation of serum proteins.

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