Peroxidative index as novel marker of hydrogen peroxide involvement in lipid peroxidation from coal dust exposure

Abstract

Nia Kania, Bambang Setiawan, Edi Widjajanto, Nurdiana Nurdiana,M. Aris Widodo4, H.M.S. Chandra Kusuma

This study aimed to elucidate whether inhalation of particulate matter 10 (PM10) coal dust change oxidative stress and peroxidative index in rats. A total of 42 Wistar male rats were randomly divided into seven groups including one control group, three groups exposed to coal dust for 14 days, and three groups exposed to coal dust for 28 days. The three groups at the 14 and 28 days exposures received a dose of coal dust equal to 6.25 mg/m3 , 12.5 mg/m3 , and 25 mg/m3 , respectively. The exposure to coal dust exposure was conducted using equipment that was designed in Pharmacology Laboratory, Medical Faculty, Brawijaya University of Malang, Indonesia. ANOVA test was used to analyze the different level of lipid peroxidation, hydrogen peroxide, and peroxidative index. The number of macrophage was significantly higher in 14 and 28 days coal dust exposure groups compared with control group. The level of lung malondialdehyde, bronchoalveolar lavage malondialdehyde, and peroxidative index were significantly higher in 14 and 28 days coal dust exposure groups compared with control group. The level of bronchoalveolar lavage hydrogen peroxide was not significantly difference in all group. We concluded that inhalation of PM10 coal dust increase lung and bronchoalveolar lavage lipid peroxidation, and also peroxidative index of rats.