Therapeutic potentiality of Kedrostis foetidissima (Jacq.) Cogn., leaf extracts on free radicals induced oxidative damage in the biological system

Abstract

Pavithra Kalaisezhiyen, V. V. Sathibabu Uddandrao, Ganapathy Saravanan, Vadivukkarasi Sasikumar

Objectives: This study was convened to evaluate the free radical scavenging activity of Kedrostis foetidissima leaf extracts. Methods: The leaves of K. foetidissima (Jacq.) Cogn. were subjected to powder and extracted with various solvents such as water, methanol, acetone, chloroform, and petroleum ether. All the extracts were subjected to nitric oxide radical scavenging assay, hydrogen peroxide scavenging assay, superoxide anion radical scavenging assay, and egg yolk lipid peroxidation (LPO) assay. Results: All the extracts of K. foetidissima effectively reduced the generation of nitric oxide from sodium nitroprusside over a concentration of 2-10 mg/ml concentration which was significant at P < 0.05, showed moderate to potent level of H2 O2 radical scavenging activity over the range of 2-10 mg/ml concentration, effectively reduced the superoxide anion radicals generated from phenazine metho-sulfate/nicotinamide adenine dinucleotide-nitroblue tetrazolium system over a concentration range of 2-10 mg/ml concentration, effectively inhibited the formation of LPO over a concentration range of 2-10 mg/ml. Conclusion: The results of this study indicated that among the five solvent extracts, the methanolic leaf extract have beneficial free radical scavenging activity and it also clearly suggested that the leaves may be used as a dietary supplement which can act as a natural antioxidant.